Del(11Hnf1b-Znhit3)11Moro

CRISPR/cas9 genome editing is used to delete the 11qC region of the mouse genome between chr11:83791635-84938224 Mb [GRCm38.p6 (mm10), NCBI Reference Sequence, NC_000077.6]. The mouse deletion matches the consensus human deletion between chr17:31.8-33.2 Mb (GRCh38.p13, NCBI Reference Sequence, NC_000017.11). The 17q12 deletion removed all genes downstream of Gm11434 and upstream of Gm33028 including Aatf, Acaca, Gm11437, Ddx52, Dhrs11, Dusp14, Ggnbp2, Lhx1, Hnf1b, Mrm1, Myo19, Pigw, Synrg and Znhit3. sgRNAs were targeted to upstream of Hnf1b and downstream of Znhit3. (J:334297)