Kcnt1^em1Trsg

Leucine codon 437 (CTC) in exon 14 was changed to phenylalanine (TTC) (NM_175462.4:c.1309C>T, p.L437F) using an sgRNA (targeting GACCTCATTCCTGCTGCTG) and an ssODN template with CRISPR/Cas9 technology. This gain-of-function mutation of the highly conserved leucine in the RCK1 domain of the encoded peptide is the equivalent of the human p.L456F mutation. (J:324580)