Tg(Bsx-icre)1Kess

Homologous recombination in bacteria was used to modify a 180 Kb BAC RP24-255N16 containing 70 Kb upstream and 110 Kb downstream of the Bsx gene. The codon-improved Cre recombinase (iCre) conntaining a nuclear localization signal was fused to the initiation codon of the genes using a PCR-based approach. This was followed by a Simian Virus 40 polyadenylation signal and a Kanamycin resistance cassette that was flanked by FRT sites for the selection of recombinant BACs. BAC recombination was designed to delete the remaining ATG-encoding exon together with 50-200 bp from the downstream intron. Mice were generated by pronuclear injection of the construct into fertilized oocytes. No line number is given. (J:330767)