Rr108735^em1Axvi

CTCF-binding sequence downstream of Tbx5 lung enhancer Rr426, located downstream of the gene, was targeted with sgRNAs (targeting GCATGCTGGGGTAAGCCCGAGGG and GCTGGGGTGCCAAGCCCAGAGGG, GGTCAGAGTCTGCCGCCCAAAGG and AAGGCCTGAGAGCGCGCCAGTGG) using CRISPR/Cas9 technology, resulting in an 1135 bp deletion (chr5:120240528-120241662 (GRCm39)). (J:335096)