CRISPR/Cas9基因编辑技术被用来在编码序列的起始密码子附近插入终止密码子,结果显示 Western blotting 和 RT-PCR 都证实了编码蛋白和转录本的缺失。(来源:J:285409)
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CRISPR/Cas9 genome editing technology was used to insert a termination codon near the start codon in the coding sequence. Western blotting and RT-PCR showed the absence of the encoded protein and transcript, respectively. (J:285409)