Slc26a5^tm1.1Jgao

The exon 3 splice acceptor site in intron 2 was modified from TAG to TGG and a loxP site flanked neomycin resistance gene cassette was inserted into intron 3. The neo cassette was removed through subsequent Cre-mediated recombination. This mutation in the 5' UTR is the equivalent of the human NM206883.2:c.53-2A>G GRCh38:Chr7:103421569 SNP rs116900495 possibly associated with nonsyndromic deafness disorder DFNB61. The mutation inactivates the splice acceptor site and activates a cryptic CAG acceptor site 11 bp downstream in exon 3. (J:324887)