CRISPR/Cas9技术通过5'-CACCACAAACACCGATGACTTGG-3的gRNA,针对Myh1和Myh4基因的第三个外显子(第一个编码外显子)设计,产生了一个单核苷酸插入在Myh4上。在这个过程中,Myh1基因也发生了单核苷酸缺失,表现为Myh1em1Ktsu(J:335578)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6J
Endonuclease-mediated
插入
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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