Fgf8^{tm3.1(cre/Esr1*)Moon}

The targeting construct inserted an IRES sequence followed by the mER:Cre:mER cDNA encoding a fusion protein with two copies of the mouse estrogen receptor (mER) flanking Cre recombinase, and a Neomycin resistance (NeoR) selection cassette flanked by Frt recombination sites into an Eag1 site in the Fgf8 gene located 36 bp 3 of the Fgf8 stop codon and 5 to the endogenous polyadenylation sequence. The linearized targeting vector was electroporated into mouse embryonic stem cells and subjected to selection. Flp-recombinase excised the NeoR. (J:301046)