Ddx41^tm1.1Ndmo

Genomic sequence for exons 11-17 and intervening introns was replaced with the following: a loxP site, cDNA sequence for exons 11-17, SV40 poly(A) signal sequences, a second loxP site, cDNA sequence for exons 11-17 where arginine codon 525 (CGT) in exon 15 was changed to histidine (CAT) (p.R525H), SV40 poly(A) signal sequences, and an FRT site flanked neomycin resistance gene cassette. The neo cassette was removed through subsequent Flp-mediated recombination. The allele expresses the wildtype peptide and only after Cre-mediated deletion of the wildtype exons 11-17 sequence will it express the mutated peptide. The mutation, in the helicase domain of the encoded peptide, affects splicing and transcriptional elongation. (J:342740)