将一个病毒性剪接接受器(SA)-loxP-诺卡因-pA-loxP-H2B-mRuby3-P2A-ERK-KTR-mClover3-WPRE-pA的 cassette插入到小鼠的Gt(ROSA)26Sor基因座。载体包含了mClover3(Addgene, #74252),mRuby3(Addgene, #74252),人类H2B(Addgene, #99267),腺病毒的剪接接受器,一个可删除的NeoR序列,ERK-KTR报告基因(Addgene, #59138),以及木鼠肝炎病毒的后转录调节元件。ERK-KTR传感器包含一个ERK1/2结合位点,连接着一个附着于荧光蛋白(FP; mClover3)的磷酸化激活核输出信号(NES)和抑制核定位序列(NLS)。来源:(J:339772)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
129S4/SvJaeSor
Targeted
插入
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--
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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