Gt(ROSA)26Sor^{em1(CAG-EGFP/Rpl10)Mgmj}

Using CRISPR/Cas9 technology, a targeting vector CAG-FSF-LSL followed by an EGFP/Rpl10 fusion reporter gene under the control of the CAG promoter (CVM enhancer/chicken ACTB promoter) was inserted into the Gt(ROSA)26Sor locus. It is an Flp- and Cre-dependent reporter. The expression of the EGFP/Rpl10 fusion reporter gene requires the removal of both LSL by Cre and FSF by Flp recombinases. (J:317381)