A vector containing ROSA26 homology arms, a CAG promoter, flox stop sequence, and Gaussia luciferase with ASARTDL tail (secreted ER calcium-monitoring protein or SERCaMP) and a FRT-flanked Neomycin gene was inserted into the Gt(ROSA)26Sor locus. The neomycin cassette was removed in the germline via FLP-mediated recombination. (J:340162)