Mtor^em1Dcf

Serine codon 2159 (TCT) in exon 46 was changed to alanine (GCT) (p.S2159A) using two sgRNAs (targeting CACCGTGGTTGGGGTCGTATGTTCC and AAACGGAACATACGACCCCAACCAC) and an ssODN template (GTTCAAAGCTCACATCCCTGGAGCTGCAGTATGTGTCCCCCAAACTTCTGATGTGCCGAGACCTTGAGTTGGCTGTGCCCGGGACATATGACCCAAACCAGCCAATCATTCGCATTCAATCCATAGCCCCGGCTTTGCAAGTCATCACATCCAAGCAGAGGCCTCGGAAGCTGACTCTGA) with CRISPR/Cas9 technology. This mutation prevents phosphorylation of the residue. (J:257513)