这个靶向载体是根据Addgene的Ai9向量构建的,从5'到3'的序列包括CMV增强子/鸡β-actin驱动的 promoter、FRT位点、loxP位点、小鼠CD40信号肽、myc标签、G5标签(五个N端甘氨酸残基)连接到Thy1.1 cDNA、polyA信号、第二个loxP位点、FLAG标签、Staphylococcus aureus转肽酶sortase A(srta)-tag的cDNA附着在一个链接上,随后是人类PDGFRB的跨膜域,接着是木村病肝炎病毒的后转录调控元件(WPRE)、另一个polyA信号,以及attB/attP引导的PGK-FRT-Neo-polyA cassette。(来源:J:334846)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
C57BL/6
Targeted
插入
--
--
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
Show/Hide columns
Phenotypes

References Literature

Title
PMID
Journal
Year
IF
No Data Found!
微信
信息比对
科研助手
使用教程
回到顶部