设计了两个导向RNA(ATTGTGGACCATTCAGCTTG和CTTAACTATGTAGTCCAGAC),目标是定位到exon 3。非同源末端连接会导致gRNAs之间序列的缺失。参考的是Klrg1转录本Klrg1-201(ENSMUST00000032207.9),用于确定exon编号以及指导序列。(来源:J:334818)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count