The Gal enhancer was targeted with sgRNAs (targeting CTCCCTGGAGCAATATGAAG and CCCGCTTTCATGGCTCCCAA) using CRISPR/Cas9 technology, resulting in a 257 bp deletion (chr19:3490919-3491175 (GRCm39)). The orthologous human enhancer is implied in male anxiety and ethanol intake. (J:334339)