NFIL3C/EBP binding site 1 in the Zeb2 enhancer, located ~165 kb upstream, was targeted with sgRNAs (targeting ATACCCATGTTACATAATTA) and an ssODN template (CAAAATAGCAGAAAACCACAGCTGCTTGAGCAGTTAACAGATATCAGTAAAATACCCATGGCGGCCGCTTAAGGATAACGTTCTTGAAGCATATGGGCTGAAGGTATTATACTCTCATTAAAACTTTA) using CRISPR/Cas9 technology, resulting in the deletion of the binding site. (J:334296)