靶向载体包含一个P2A序列,接着是eGFP/CreERT融合蛋白以及一个FRT引导的PGK-neomycin筛选元件。构建被插入在acathe-scute家族转录因子3(Ascl3)基因的终止密码子上游。随后通过Flp重组酶移除了neo选择性元件。(来源:J:101977)
Basic Information
(129S6/SvEvTac x C57BL/6NCrl)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count