Atoh1^{tm2.1(cre/ERT2,-CD2)Dwi}

The targeting construct was designed to introduce a loxP site into 5'UTR of Atoh1, followed by the hyperactive phosphomutant (9 amino acids S/T to A changes) Atoh1 sequence Atoh1(9S/T-A) merged to the P2A-CreERT2-hCD2 then to a neomycin cassette inserted such that the 3'UTR was not disrupted. Following successful germline transmission, the mice heterozygous for the targeting construct were crossed onto PGK-Cre line in order to remove both the neo-selection cassette and the endogenous Atoh1 locus at the same time. (J:271399)