A loxP site was inserted upstream of the sequence and an FRT flanked pgk-neo cassette and loxP site were inserted downstream of the sequence via homologous recombination. Flp-mediated recombination removed the neo cassette. (J:314799)
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A loxP site was inserted upstream of the sequence and an FRT flanked pgk-neo cassette and loxP site were inserted downstream of the sequence via homologous recombination. Flp-mediated recombination removed the neo cassette. (J:314799)