Atoh1 enhancer E2 was targeted with sgRNAs (targeting GAAGTTTCACTGGACACTGGAGG and AATGACAGAGGTCTTGACAGAGG) using CRISPR/Cas9 technology, resulting in a deletion-insertion with an 890 bp deletion (chr6:64750197-64751086 (GRCm39)), including the enhancer, and a 1 bp insertion (C). (J:332270)