The transgene contains the mouse Sox2 genomic DNA (BAC clone RP23-4D1), HA (hemagglutinin) tag sequence, Optimized luciferase cDNA (synthetic), yeast Frt (flippase recombination target) site, and Phage P1 loxP site. Line 1 expresses a luc2-Sox2 fusion protein. (J:203419)