Comt^tm1.1Tamm

A construct containing a modified version of exon 2 with two point mutations (ATGCTG to GAGCTC) in the P2 promoter and a floxed pgk-neo cassette in intron 2 was inserted replacing the endogenous sequence via homologous recombination. One of the point mutations in exon 2 converts methionine to glutamic acid while the second is silent.Cre mediated recombination then removed the neo cassette. The mutant gene expresses only the soluble isoform, expression of the membrane-bound isoform is absent. (J:333577)