CRISPR-targeting inserted into the locus a CAG promoter driving expression of a FLAG-tag Streptococcus pyogenes dead Cas9 (dCas9) and FRT-flanked IRES and EGFP reporter gene. Cre-mediated recombination removed the selection cassette. Flp-mediated recombination removed the FRT-flanked EGFP cassette. (J:258204)