通过CRISPR/Cas9技术,将编码CreERT2的cDNA,后面跟着polyadenylation序列,插入Hey2基因的最后一个编码外显子。在导向载体中包含了一个P2A肽序列,以连接Hey2的编码区域(在终止密码子之前),从而实现Hey2和CreERT2蛋白的共同表达。(来源:J:263128)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Endonuclease-mediated
插入
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1
1
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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