The Zbtb7b silencer was targeted with an ssODN template, coding for a version of the silencer sequence where a 40 bp sequence of a highly conserved noncoding element (HCNE; located at the 3' end) containing two conserved Runx binding motifs is moved to the 5' end, using TALEN technology (ZNF target sequences (capitalized) ACCGCTACCCTAACCcataaCTGGAAGGGGTTTAG). (J:333471)