CRISPR/Cas9技术通过sgRNA5'-5'GGAATGGCCTGTGGTTGACT-3(低尾部的g表示5个错配)产生了携带exon 2所有读框内终止密码子的实验鼠。在基因组位置GRCm38/mm10 chr2:128,729,258插入的终止子序列是:5'-cgcTAAgTGAcTGAccaccgc-3(终止子以大写表示,不包括邻近同源序列)。来源:(J:288823)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6N
Endonuclease-mediated
插入
--
1
4
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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