The L1L2_Bact_P cassette R3R4_pBR_DTA+_Bsd_amp backbone from IKMC project 77718 was used to generate this allele. A cassette containing FRTEngrailed2 exon IRESLacZloxPneoFRTloxP was inserted in intron 7, and a loxP was inserted in intron 10. The lacZ and neo were removed via flp-mediated recombination leaving exons 8-10 floxed. (J:333076)