F2rl1^em1Wmrf

Lysine codon 36 (AAA) in exon 2 was changed to aspartic acid (GAA) (p.K36E) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation results in the resistance of the encoded peptide to skin-expressed serine proteases while remaining sensitive to cleavage by F10. (J:333152)