靶向载体通过FLP剔除系统(FLEx)构建,包含从5'至3'的序列:lox2272、loxP座,一个包含Ank3最后一个外显子的FLEx插件(ENSMUSE00001264329位于ENSMUST00000182992.8),一个FRT引导的neo cassette,pGHpA序列,以及反向排列的EGFP报告基因,后面跟着lox2272和loxP座,这些插入在内源性最后一个外显子下游。通过FLP介导的重组,neo cassette被剔除了。(来源:J:333091)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129S6/SvEvTac x C57BL/6NCrl)F1
Targeted
插入
--
1
2
--

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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