CRISPR/cas9 genome editing uses guide RNAs (AAAAGCTTCTGACGTACCACagg and AAGTAACAGCGGAGCGCTTTtgg, PAM sequences indicated by lowercase letters) to delete exons 2 to 6. Il1a transcript from Mus musculus interleukin 1 alpha (Il1a); NCBI Reference Sequence: NM_010554.4, was used as reference for the exon numbering and the guide/donor sequences. (J:332933)