Tg(Igkv3-5*-MYC)DLoxP11Plbe

The mouse promoter drives B cell-specific expression of the human MYC coding sequence (exons 2 and 3; Genbank vector OQ428542). A codon mutation generates a STOP codon at the third codon (TCG to TAG) and prevents expression of MYC. The codon substitution created a DGYW motif, a known preferential target sequence for somatic hypermutation, that can activate MYC translation following sporadic reversion. This vector lacks the loxP site found in Tg(Igkv3-5*-MYC)24Plbe. In line 11, the transgene inserted between chr6:4020486-4025566 with deletion of chr6:3856340-4020485 (that includes Tfpi2, Gngt1 and Gng11) and deletion of chr6:4025566-4279599 (that includes Bet1). (J:131912)