Gt(ROSA)26Sor^{em1(Btg1)Meln}

A targeting vector inserted a loxp flanked stop cassette in intron1 of the Rosa26 locus followed immediately by a wild-type murine Btg1 cDNA-T2A-Luciferase sequence. The allele was generated by introducing a single-nucleotide substitution to revert the Q36H mutation of the cDNA sequence in Gt(ROSA)26Sortm1(Btg1*)Meln to a wild-type sequence by CRISPR-Cas9-mediated genome targeting. (J:332796)