Pstpip2^em5Tbrd

CRISPR/Cas9 technology, using an sgRNA (targeting ACTTCTTCCGGAATGCACTG) and an ssODN template, generated a 116 bp deletion (chr18:77960990-77961105 (GRCm39)) encompassing part of the exon coding W323 together with a part of the preceding intron, resulting in the complete loss of expression as confirmed by Western blot analysis. (J:332442)