A CAG-loxP-Stop-loxP-V5-Map2k5 cDNA containing a serine to aspartate substitution at amino acid 311 (p.S311D) and a threonine to aspartate substitution at amino acid 315 (p.T315D)-polyA cassette was inserted into intron 1 via CRISPR/Cas9 technology. (J:328655)