Tbc1d25^em1(IMPC)Tcp

This allele was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of TGAGCCCAGATTACAGGGCG targeting the 5' side and CATTGGGGTTGGAGCGTTGG targeting the 3' side of a critical region (ENSMUSE00001238169, ENSMUSE00001307373 & ENSMUSE00000771621) along with a single-strand oligonucleotide encoding a Bxb1 attB site. This resulted in a 2,152-bp deletion of ChrX from 8,020,930 to 8,023,081 (GRCm39) with an insertion of a Bxb1 attB sequence (GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGATCATACACCTT). (J:265051)