Slc27a3^em1(IMPC)Tcp

This allele was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of AGCCTCAGCAAACACGAGTA targeting the 5' side and CCTCCCGCTGGGCCGCCTCT targeting the 3' side of a critical region (ENSMUSE00001308785) along with a single-strand oligonucleotide encoding a Bxb1 attB site. This resulted in a 297-bp deletion of Chr3 from 90,294,858 to 90,295,154 (GRCm39) with an insertion of a Bxb1 attB sequence (GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGATCATACACCTT). (J:265051)