Bloc1s5^em1(IMPC)Tcp

This allele was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of AAGGGTTAAGTCACACTCCG targeting the 5' side and GTAGTAGGCCATTAACAACT targeting the 3' side of a critical region (ENSMUSE00000291432) along with a single-strand oligonucleotide encoding a Bxb1 attB site. This resulted in a 160-bp deletion of Chr13 from 38,814,930 to 38,815,089 (GRCm39) with insertion of a Bxb1 attB sequence (GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGATCATACACCGG). (J:265051)