The Pitx2 AF (atrial fibrillation)-related enhancer, located upstream of the gene, was targeted with sgRNAs (targeting GTATTTCATCTAAGGCACAG and CATGGTCACAGTCAATGTGC) using CRISPR/Cas9 technology, resulting in an ~18.6 kb deletion. The deleted sequence contains predicted enhancer Rr79124. (J:281534)