Dnmt3a^em2Mago

CRISPR/Cas9 technology deleted the third coding exon (exon 4; 268 bp). Deleting exon 4 leads to a frameshift and premature termination, resulting in the knock-out of the full-length isoform 1 (Dnmt3a1). Western blot analysis confirmed the absence of isoform 1 protein in thymus and brain without affecting expression of isoform 2. (J:331942)