通过同源重组,一个包括5到8号外显子的基因区域被一个pgk-neo cassette替换了。 Western blot结果显示,纯合小鼠的耳蜗中没有检测到蛋白表达。(来源:J:324886)
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A genomic region including exons 5 through 8 was replaced with a pgk-neo cassette via homologous recombination. Western blot analysis confirmed the absence of protein expression in cochlea from homozygous mice. (J:324886)