CRISPR-cas9 meidated recombination using guide RNAs (gRNA1:GTGCCTCATTGGGAGCACCG; gRNA2:CTGTACAGTCTCACACCAAG) targeting exons 2 and 3 created a deletion removing part of exon 2 and all of exon 3. Western blot and qPCR analyses confirmed the absnece of expression in homozygous mice. (J:329548)