A piggyBac transposon vector bearing a human UBC promoter-driven loxP-lacZ-stop-loxP cassette 5 of a FLAG-tagged mouse Trim24 cDNA was injected into FVB/NCr zygotes along with transposase RNA. The flag-tagged mouse Trim24 transposon integrated as a single copy in the q-arm of chromosome 1 without disrupting any known genes or regulatory regions. (J:311171)