CRISPR/cas9通过同源定向修复(HDR)技术,利用两个导向RNA(CACCgTACACGCTATATTTGTGCTA和AAACTAGCACAAATATAGCGTGTAc,以及CACCgTACAGACTGTAGTTTAGCTT和AAACAAGCTAAACTACAGTCTGTA),在CGI(位于区域5'端的 CpG 岛)附近插入loxP序列。来源:(J:331751)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6 x DBA/2)F1
Endonuclease-mediated
插入
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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