Clcn2^tm4.1Tjj

Exons 2 and 3 were flanked by loxP sites and a neomycin-resistance cassette flanked by FRT sites was inserted between exons 1 and 2. To prevent missplicing, a putative splice acceptor site downstream of the neomycin cassette was mutated (gt to ca) and an additional EcoRV-site was introduced adjacent to the second loxP site to aid in Southern blot detection. The neomycin cassette was removed via flp-mediated recombination. (J:331730)