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中文
通过Red/ET重组技术，对含有小鼠Mir22基因的BAC克隆CH29-40010进行了操作。在exon 1的5'上游区域插入了loxP，然后在exon 1的3'下游区域引入了第二个loxP位点，使用了PGK-FRT-Neo-FRT-loxP载体。靶向的ES细胞通过表达flp重组酶的质粒进行了再电转，以去除Neo抗性基因，使得exon 1区域去除了。在生殖细胞系中，通过Cre介导的重组，exon 1被删除。RT-qPCR结果显示，肝脏、阴囊白色脂肪组织和股四头肌的成熟miR-22-3p量检测不到。(来源：J:331713)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
