The transgene insert contains the following elements: 400 bp of Trav promoter, Xbal-BamHI restriction fragment from the AN14.4 hybridoma (containing Trav11/Traj14 rearranged segments coding for receptor for pigeon cytochrome c and Ek), the T cell receptor alpha constant gene segment (including 1.1 kb of the preceding Traj-Trac intron), Tcra enhancer Rr308, and Tcra locus control region (LCR) Rr312 (containing DNAseI hypersensitivity sites HS2-6). (J:330515)