Vps45^{tm1c(EUCOMM)Hmgu}

The L1L2_Bact_P cassette was inserted at position 95955412 of Chromosome 3 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 95956717. The critical exon 4 is thus flanked by loxP sites. The neomycin resistance gene was removed via flp-mediated recombination generating a conditional ready (floxed) allele. (J:331313)