Letmd1^em1Kshi

CRISPR/Cas9 technology in combination with mouse oviduct electroporation (i-GONAD; improved-Genome editing via Oviducal Nucleic Acids Delivery) deleted exons 3 to 7, causing a frameshift and inducing a stop codon, resulting in a truncated protein of 104 aa excluding the transmembrane domain a majority of the LETM1 domain. Western blot analysis confirmed the absence of protein. (J:318013)