在exon 1上游插入了loxP位点,通过同源重组在exon 2下游插入了loxP和FRT标记的neo cassette。通过Flp介导的重组,neo cassette被移除,exons 1和2被留下了门控区域。随后,Cre介导的重组去除掉了exons 1和2。(来源:J:242006)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count